- 1(current)
Q. No.
Which of the following is/are not indirect method/ s of gene transfer?
| a. | Bacteriophages |
| b. | Plasmids |
| c. | Mobile genetic elements (transposons) |
| d. | Chemical mediated gene transfer |
| e. | Electroporation |
Options
1. a & b
2. b & c
3. d & e
4. c, d & e
To transform a bacterial cell with recombinant DNA, which of the following is required?
| 1. | 90°C temperature |
| 2. | Divalent calcium ions |
| 3. | Selectable marker |
| 4. | Restriction enzyme digested DNA |
| 1. | It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells. |
| 2. | 'Ti' plasmid from Agrobacterium tumefaciens used for gene transfer is not pathogenic to plant cells. |
| 3. | It transforms normal plant cells into tumor cells. |
| 4.. | It delivers 'T-DNA' into plant cell. |
Following are vectorless gene transfer except one. Mark the except one
| 1. | Micro injection | 2. | Electroporation |
| 3. | Cosmid | 4. | Biolistic |
Method of gene transfer that involves use of electrical impulses of high field strength to increase permeability of membrane by creating transient pores in it, is
| 1. | Microinjection |
| 2. | Electroporation |
| 3. | Biolistics |
| 4. | Lipofection |
All the following are transformation procedures except
1. Microinjection
2. Biolistics
3. Spooling
4. Electroporation
Genetically engineered plants are generated in laboratory by altering their genetic makeup. This is done by adding one or more genes to a plant’s genome by
1. Biolistic method or Agrobacterium tumefaciens mediated transformation
2. DNA injection or heat shock method
3. Fusing liposomes to intact plant cells
4. Bacillus thuringiensis mediated transformation
| 1. | Silver | 2. | Copper |
| 3. | Zinc | 4. | Tungsten or gold |
- 1(current)
Q. No.
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