| 1. | Enzyme Linked Immuno-Sorbent Assay (ELISA) technique |
| 2. | Recombinant DNA Technology |
| 3. | Serum and Urine analysis |
| 4. | Polymerase Chain Reaction (PCR) technique |
| 1. | Mature insulin molecule consists of three polypeptide chains - A, B and C. |
| 2. | Insulin is synthesized as prohormone which contains extra stretch of C-peptide. |
| 3. | C-peptide is not present in mature insulin molecule. |
| 4. | Polypeptide chains A and B are linked to disulphide bridges. |
| List-I | List-II | ||
| (A) | Gene therapy | (I) | Separation of DNA fragments |
| (B) | RNA interference | (II) | Diagnostic test for AIDS |
| (C) | ELISA | (III) | Cellular defense |
| (D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
| Options: | (A) | (B) | (C) | (D) |
| 1. | IV | I | II | III |
| 2. | IV | II | III | I |
| 3. | IV | III | II | I |
| 4. | IV | III | I | II |
ds RNA can be used during which one of the following?
| 1. | Gene sequencing | 2. | Polymerase Chain Reaction |
| 3. | Gene silencing | 4. | Autoradiography |
| 1. | Genetically engineered lymphocytes are not immortal cells. |
| 2. | Retroviral vector is introduced into these lymphocytes. |
| 3. | Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages |
| 4. | Lymphocytes from patient's blood are grown in culture, outside the body. |
| (a) | Pro-hormone insulin contain extra stretch of C-peptide |
| (b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
| (c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
| (d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
| (e) | Some patients develop allergic reactions to the foreign insulin. |
Genetically engineered insulin for human is produced from:
1. Escherichia coli
2. Psuedomonas putida
3. Bacillus thuringiensis
4. Rhizobium meliloti
Give below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In human beings, insulin is synthesized as a pro-hormone which needs to be processed before it becomes fully mature and functional. |
| Reason (R): | The extra stretch of C-peptide is to be removed from A and B peptide chain of insulin. |
In the light of the above statements, Choose the most appropriate answer from the option given below :
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
| Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
| Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 1. | Human protein \(\alpha\) –1–antitrypsin. |
| 2. | Human alpha–lactalbumin. |
| 3. | Human insulin–like growth factor. |
| 4. | Human enzyme Adenosine Deaminase (ADA). |