Q. No.Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
| Assertion (A): | Restriction enzyme is a type of endonuclease. |
| Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
| 1. | High temperature | 2. | Two sets of Primers |
| 3. | Taq DNA polymerase | 4. | Deoxynucleotides |
Identify the properties of a good vector used in rDNA technology
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only
2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c) , (d) and (e) only
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of the palindromic site. |
| 1. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 3. | (A) is correct but (R) is not correct. |
| 4. | (A) is not correct but (R) is correct. |
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
| List-I | List-II | ||
| (a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
| (b) | Gene therapy | (ii) | Used for transfer of gene |
| (c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
| (d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
| (a) | (b) | (c) | (d) | |
| 1. | (ii) | (i) | (iv) | (iii) |
| 2. | (i) | (iii) | (ii) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (ii) | (iii) | (iv) | (i) |
| 1. | (a) Cellulase (b) Protease | 2. | (a) Cellulase (b) Chitinase |
| 3. | (a) Chitinase (b) Lipase | 4. | (a) Cellulase (b) Lipase |
| 1. | Cosmids | 2. | Plasmids |
| 3. | Nucleus | 4. | Nucleoid |
© 2025 GoodEd Technologies Pvt. Ltd.