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Q. No.Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
| Assertion (A): | Restriction enzyme is a type of endonuclease. |
| Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Restriction Enzymes - Main Enzymes |
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Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Selection of Recombinant Transformants |
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
Subtopic: General Design of an rDNA experiment |
66%
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
| 1. | High temperature | 2. | Two sets of Primers |
| 3. | Taq DNA polymerase | 4. | Deoxynucleotides |
Subtopic: Polymerase Chain Reaction: PCR |
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Identify the properties of a good vector used in rDNA technology
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only
2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c) , (d) and (e) only
Subtopic: Cloning Vector |
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Given below are two statements one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the correct answer from the options given below :
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of the palindromic site. |
| 1. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 3. | (A) is correct but (R) is not correct. |
| 4. | (A) is not correct but (R) is correct. |
Subtopic: Restriction Enzymes - Main Enzymes |
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Separation and Isolation of DNA fragments |
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Match List-I with List-II :
Choose the correct answer from the options given below :
| List-I | List-II | ||
| (a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
| (b) | Gene therapy | (ii) | Used for transfer of gene |
| (c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
| (d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
| (a) | (b) | (c) | (d) | |
| 1. | (ii) | (i) | (iv) | (iii) |
| 2. | (i) | (iii) | (ii) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (ii) | (iii) | (iv) | (i) |
Subtopic: Process of Biotech | Transforming Plant & Animal Cell |
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The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :
| 1. | (a) Cellulase (b) Protease | 2. | (a) Cellulase (b) Chitinase |
| 3. | (a) Chitinase (b) Lipase | 4. | (a) Cellulase (b) Lipase |
Subtopic: Separation and Isolation of DNA fragments |
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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
| 1. | Cosmids | 2. | Plasmids |
| 3. | Nucleus | 4. | Nucleoid |
Subtopic: Process of Biotech |
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