- 1(current)
Q. No.Identify a character that is not desirable in a cloning vector:
| 1. | an inactive promoter |
| 2. | an origin of the replication site |
| 3. | selectable markers such as genes for antibiotic resistance |
| 4. | one or more unique restriction endonuclease sites |
The trait ‘resistance to certain antibiotics’ is seen in some bacteria due to the presence in them of:
| 1. | Inclusion bodies |
| 2. | Membranous extensions of plasma membrane |
| 3. | Plasmid DNA |
| 4. | Glycocalyx |
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
| 1. | Plastid | 2. | Bacteriophages |
| 3. | BAC | 4. | Plasmid |
A piece of DNA is inserted within the gene of beta-galactosidase in the E.coli plasmid. X-gal is added to the medium during screening. The colonies of recombinant transformants:
| 1. | will show an increase in growth |
| 2. | will not be able to survive |
| 3. | will give a deep blue color |
| 4. | will not produce any color |
In the diagram showing the plasmid pBR322, the recognition sequence of the restriction enzyme BamHI is shown by the number:
| 1. | 1 | 2. | 2 |
| 3. | 3 | 4. | 4 |
A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
| 1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
| 2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
| 3. | Both will select only transformants. |
| 4. | Both will select only recombinants. |
- 1(current)
Q. No.
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