Which of the following would NOT be a useful selectable marker?

1.  A gene encoding a protein that degrades the antibiotic ampicillin.
2.  A gene encoding a protein that allows the cell to synthesize histidine.
3.  A gene encoding a protein that is an essential structural component of the cell.
4.  All of these are useful selectable markers.
Subtopic:  Selectable Markers: I | Selectable Markers: II | Selectable Markers |
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You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline-resistant gene. You plate your transformed bacteria on media containing tetracycline and X-gal. Which of the following results would indicate a clone with recombinant plasmids?

1.  A clone which did not grow on the tetracycline plates
2.  A white colony on the tetracycline plates
3.  A blue colony on the tetracycline plates
4.  A red colony on the tetracycline plates

Subtopic:  Selectable Markers: III | Selectable Markers |
 58%
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Consider the following statements: 

I:  Ori is responsible for the copy number of the linked DNA.
II:  Transformation is a process through which a piece of DNA is introduced into a host bacterium. 
III:  Bacteriophages have very high copy numbers of their genome within the bacterial cell.

Which of the above statements are true?

1.  I and II only 2.  I and III only
3.  II and III only  4.  I, II, and III
Subtopic:  Tools: Vectors: I | Selectable Markers: I | Selectable Markers | Tools |
 79%
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