Q. No.A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
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For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
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The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
| 1. | Salmonella typhimurium | 2. | Escherechia coli |
| 3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
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In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
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The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:
| 1. | depends on a particular or sequence of the main chromosomal DNA |
| 2. | is possible due to promoter sequence present in the insert |
| 3. | cannot occur without telomeric sequences |
| 4. | is independent of the control of chromosomal DNA |
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
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A cloning vector must:
| 1. | be able to maintain a very high copy numbers of their genome in the host cells |
| 2. | contain elements such as promoter and ribosome binding sites |
| 3. | be engineered to carry a large number of recognition sequences for common restriction enzymes |
| 4. | be able to replicate within host cell independent of the control of chromosomal DNA |
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What is true for the plasmids used as cloning vectors in recombinant procedures?
| 1. | Ori is responsible for controlling the copy number of linked DNA |
| 2. | The bacteria lacking R plasmids are resistant to antibiotics |
| 3. | The vector must have many sites for the same restriction enzyme |
| 4. | They can carry largest inserts amongst the available cloning vectors |
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If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its number:
| 1. | more than the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 2. | equal to the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 3. | equal to the number of copy number of bacteriophages in the cells and less than the copy number of plasmid in the cell. |
| 4. | less than the number of copy number of bacteriophages in the cells and more than the copy number of plasmid in the cell. |
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