Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
A. | Cut the DNA with blunt end |
B. | Cut the DNA with sticky end |
C. | Recognises a specific palindromic sequence. |
D. | Cut the DNA between the base G and A where encounters the DNA sequence 'GAATTC' |
E. | Exonuclease |
In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'
Statement I: | Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. |
Statement II: | Restriction endonucleases cut the DNA strand a little away from the centre of the Palindromic site. |
Given below are two statements: One is labeled as Assertion (A) and the other is labeled as Reason (R)
Assertion (A): | Restriction enzyme is a type of endonuclease |
Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
1. | Both (A) and (R) are true and (R) correctly explains (A). |
2. | Both (A) and (R) are true and (R) does not correctly explain (A). |
3. | (A) is true, (R) is false. |
4. | (A) is false, (R) is true. |
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
Identify the wrong statement with regards to restriction enzyme:
1. | They cut the strength at palindromic sites. |
2. | They are useful in genetic engineering. |
3. | Sticky ends can be joined by using DNA ligases. |
4. | Each restriction enzyme functions by inspecting the length of a DNA sequence. |