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Q. No.Which of the following statements are not true regarding restriction endonucleases?
Choose the answer from the options given below:
| A. | They are called molecular scissors. |
| B. | These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. |
| C. | They cut the DNA only at the centre of the palindromic sites. |
| D. | They remove nucleotides only from the ends of DNA fragments. |
| E. | They recognise specific palindromic base-pair sequences. |
Choose the answer from the options given below:
| 1. | A and B only |
| 2. | D and E only |
| 3. | C and D only |
| 4. | A and E only |
Subtopic: Restriction Enzymes - Main Enzymes |
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Match List-I with List-II:
Choose the correct answer from the options given below :
1. A-II, B-I, C-IV, D-III
2. A-I, B-IV, C-III, D-II
3. A-II, B-III, C-I, D-IV
4. A-I, B-II, C-IV, D-III
| List-I | List-II | ||
| A. | Genetically modified organism | I. | Agrobacterium tumefaciens |
| B. | Thermostable DNA | II. | Bt cotton |
| C. | Ti plasmid | III. | Thermus aquaticus |
| D. | pBR322 | Iv. | Eschericihia coli |
Choose the correct answer from the options given below :
1. A-II, B-I, C-IV, D-III
2. A-I, B-IV, C-III, D-II
3. A-II, B-III, C-I, D-IV
4. A-I, B-II, C-IV, D-III
Subtopic: Process of Biotech |
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Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
1. Denaturation → Annealing → Extension
2. Denaturation → Extension → Annealing
3. Extension → Annealing → Denaturation
4. Annealing → Denaturation → Extension
1. Denaturation → Annealing → Extension
2. Denaturation → Extension → Annealing
3. Extension → Annealing → Denaturation
4. Annealing → Denaturation → Extension
Subtopic: Polymerase Chain Reaction: PCR |
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Which of the following statements are correct with respect to DNA separation, isolation and visualization ?
Choose the correct answer from the options given below:
1. A and D only
2. B and D only
3. B and C only
4. A and B only
| A. | The cutting of DNA is done by molecular scissors. |
| B. | The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. |
| C. | The separated DNA fragments can be seen without staining when exposed to UV light. |
| D. | The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. |
Choose the correct answer from the options given below:
1. A and D only
2. B and D only
3. B and C only
4. A and B only
Subtopic: Separation and Isolation of DNA fragments |
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Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards :
| 1. | Gentamycin |
| 2. | Ampicillin and tetracycline |
| 3. | Tetracycline |
| 4. | Ampicillin |
Subtopic: Cloning Vector |
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In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Subtopic: Cloning Vector |
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Polymerase chain reaction (PCR) amplifies DNA following the equation.
| 1. | \(2n+1\) | 2. | \(2N^2\) |
| 3. | \(N^2\) | 4. | \(2^n\) |
Subtopic: Polymerase Chain Reaction: PCR |
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Identify the part of a bio-reactor which is used as a foam braker from the given figure.
| 1. | D | 2. | C |
| 3. | A | 4. | B |
Subtopic: Bioreactors/Downstream Processing |
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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method:
Given below are two statements about this method:
| Statement I: | The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. |
| Statement II: | The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. |
| 1. | Statement I is correct; Statement II is incorrect |
| 2. | Statement I is incorrect; Statement II is correct |
| 3. | Both Statement I and Statement II are correct |
| 4. | Both Statement I and Statement II are incorrect |
Subtopic: Selection of Recombinant Transformants |
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Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
| 1. | D and E only | 2. | B and C only |
| 3. | C and D only | 4. | A and B only |
Subtopic: Tools |
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