- 1(current)
Q. No.Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
Subtopic: Polymerase Chain Reaction: PCR |
86%
From NCERT
NEET - 2024
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic: Polymerase Chain Reaction: PCR |
90%
From NCERT
NEET - 2023
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R)
In the light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | Polymerase chain reaction is used in DNA amplification. |
| Reason (R): | The ampicillin resistant gene is used as a selectable marker to check transformation. |
| 1. | (A) is not correct but (R) is correct. |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 4. | (A) is correct but (R) is not correct. |
Subtopic: Selectable Markers: I | Polymerase Chain Reaction: PCR |
77%
From NCERT
NEET - 2022
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Subtopic: Polymerase Chain Reaction: PCR |
67%
From NCERT
NEET - 2022
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
1. Extension, Denaturation, Annealing
2. Annealing, Denaturation, Extension
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing
Subtopic: Polymerase Chain Reaction: PCR |
85%
From NCERT
NEET - 2021
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
| 1. | Denaturation | 2. | Ligation |
| 3. | Annealing | 4. | Extension |
Subtopic: Polymerase Chain Reaction: PCR |
87%
From NCERT
NEET - 2021
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
Subtopic: Polymerase Chain Reaction: PCR | Bioreactors/Downstream Processing | Separation and Isolation of DNA fragments |
87%
From NCERT
NEET - 2020
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
Links
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
The Taq polymerase enzyme is obtained from:
1. Thiobacillus ferrooxidans
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
Subtopic: Polymerase Chain Reaction: PCR |
95%
From NCERT
NEET - 2016
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Which one is a true statement regarding DNA polymerase used in PCR?
| 1. | It is used to ligate introduces DNA in recipient cells |
| 2. | It serves as a selectable marker |
| 3. | It is isolated from a virus |
| 4. | It remains active at high temperatures |
Subtopic: Polymerase Chain Reaction: PCR |
78%
From NCERT
AIPMT - 2012
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
Hints
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
- 1(current)
Q. No.
© 2025 GoodEd Technologies Pvt. Ltd.