| A. | Release of products of the reaction |
| B. | Binding of substrate to the active site of the enzyme |
| C. | Formation of enzyme-substrate complex |
| D. | Alteration in the shape of the enzyme |
| E. | Enzyme free to bind another molecule of substrate |
| I: | Almost all enzymes are proteins. |
| II: | Some nucleic acids can behave like enzymes. |
| III: | No enzyme in living organisms can function above 40 °C. |
| IV: | Enzymes are the reason that chemicals react with each other. |
| V: | Enzymes are not sensitive to pH in their environment. |
The substrate has to go through a higher energy state or transition state during its conversion to a product in:
| I: | Exothermic spontaneous reactions |
| II: | Endothermic reactions |
| III: | Enzyme catalyzed reactions |