| A. | Release of products of the reaction | 
| B. | Binding of substrate to the active site of the enzyme | 
| C. | Formation of enzyme-substrate complex | 
| D. | Alteration in the shape of the enzyme | 
| E. | Enzyme free to bind another molecule of substrate | 
| I: | Almost all enzymes are proteins. | 
| II: | Some nucleic acids can behave like enzymes. | 
| III: | No enzyme in living organisms can function above 40 °C. | 
| IV: | Enzymes are the reason that chemicals react with each other. | 
| V: | Enzymes are not sensitive to pH in their environment. | 
The substrate has to go through a higher energy state or transition state during its conversion to a product in:
| I: | Exothermic spontaneous reactions | 
| II: | Endothermic reactions | 
| III: | Enzyme catalyzed reactions |