1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
2. | The process of extraction of separated DNA strands from gel is called elution. |
3. | The separated DNA fragments are stained by using ethidium bromide. |
4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
(a) | Agarose is a natural polymer obtained from sea-weed. |
(b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
(c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
(d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
1. | (a) and (b) only | 2. | (a), (b) and (c) only |
3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
1. | Dark red bands | 2. | Bright blue bands |
3. | Yellow bands | 4. | Bright orange bands |
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1. | Histones | 2. | Polysaccharides |
3. | RNA | 4. | DNA |
In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Match the following techniques or instruments with their usage:
(a) | Bioreactor | (i) | Separation of DNA fragments |
(b) | Electrophoresis | (ii) | Production of large quantities of products |
(c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
(d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Spooling is:
1. | Amplification of DNA |
2. | Cutting of separated DNA bands from the agarose gel |
3. | Transfer of separated DNA fragments to synthetic membranes |
4. | Collection of isolated DNA |