Q. No.To transform a bacterial cell with recombinant DNA, which of the following is required?
1.
90°C temperature
2.
Divalent calcium ions
3.
Selectable marker
4.
Restriction enzyme digested DNA
In rDNA technology or genetic engineering elution means :-
| 1. | Remove the DNA from centrifuge tube after centrifugation |
| 2. | Separation of the recombinant protein from recombinant cell |
| 3. | Insertion of recombinant DNA into host cell |
| 4. | The separated band of DNA are cut out from the gel and extracted from the gel piece |
Modern biotechnology consist:-
1. Microbiology
2. Tissue culture
3. Genetic engineering
4. All the above
The construction of the first recombinant DNA by linking antibiotic resistance gene with a native plasmid of Salmonella typhimurium was done by
| 1. | Arber and Nathans |
| 2. | Kary Mullis |
| 3. | Herbert Boyer and Stanley Cohen |
| 4. | Smith and Wilcox |
'Insertional inactivation' is used for
| 1. | Selection of recombinants |
| 2. | Producing pest resistant crops |
| 3. | Enhancing nutritional value of food |
| 4. | Reducing post harvest losses |
Given below are the three basic steps in genetically modifying an organism
| a. | Introduction of the identified DNA into the host. |
| b. | Maintenance of introduced DNA in the host and transfer of the DNA to its progeny. |
| c. | Identification of DNA with desirable genes. |
Choose the option with respect to correct sequence of the given steps
1. a → c → b
2. c → b → a
3. c → a → b
4. b → a → c
Method of gene transfer that involves use of electrical impulses of high field strength to increase permeability of membrane by creating transient pores in it, is
| 1. | Microinjection |
| 2. | Electroporation |
| 3. | Biolistics |
| 4. | Lipofection |
The figure below shows three steps (A, B, C) of polymersase chain reaction (PCR). Select the correct identification together with what it represents
1. B - Denaturation at a temperature of about 98°C separating the two DNA strands
2. A - Denaturation at a temperature of about 60°C
3. C - Extension in the presence of heat stable DNA polymerase
4. A - Annealing with two sets of primer
Which of the following statements is incorrect with respect to polymerase chain reaction?
| 1. | Repeated amplification is achieved by the use of thermostable DNA polymerase |
| 2. | Thermostable DNA polymerase is isolated from a bacterium, Thermus aquaticus |
| 3. | Each cycle in PCR has three steps, first is primer annealing, second is denaturation and third is extension of primers |
| 4. | In polymerase chain reaction, multiple copies of gene of interest are synthesized in vitro using two sets of primers and enzyme DNA polymerase |
Following are the steps in the formation of recombinant DNA by action of a restriction endonuclease. Which one of the given option incorrectly identifies the steps, components labelled as A, B, C, and D?
| 1. | A - Exonuclease cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA |
| 2. | B - Sticky ends are formed. |
| 3. | C - The DNA fragments join at sticky ends by DNA ligase. |
| 4. | D - Restriction enzyme Eco RI cuts the strand of DNA a little away from centre of the palindromic sites, but between the same two bases on the opposite strands |
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