Q. No.RNA interference is used for which of the following purposes in the field of biotechnology?
1.
to develop a plant tolerant to abiotic stresses
2.
to develop a pest-resistant plant against infestation by nematode
3.
to enhance the mineral usage by the plant
4.
to reduce post-harvest losses
The laws and rules to prevent unauthorized exploitation of bio-resources are termed as:
1. Biopatenting
2. Bioethics
3. Bioengineering
4. Biopiracy
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
1. Purification of isolated protein
2. Detection of gene mutation
3. Molecular diagnosis
4. Gene amplification
When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:
1. Molecular diagnosis
2. Safety testing
3. Biopiracy
4. Gene therapy
Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
| 1. | mutated gene does not appear on a photographic film as the probe has no complementarity with it. |
| 2. | mutated gene does not appear on a photographic film as the probe has complementarity with it. |
| 3. | mutated gene partially appears on a photographic film. |
| 4. | mutated gene completely and clearly appears on a photographic film. |
With regard to insulin, choose the correct options:
| a: | C-peptide is not present in mature insulin. |
| b: | The insulin produced by rDNA technology has C-peptide. |
| c: | The pro-insulin has C-peptide. |
| d: | A-peptide and B-peptide of insulin are interconnected by disulphide bridges. |
Choose the correct answer from the options given below:
| 1. | (a), (c) and (d) only | 2. | (a) and (d) Only |
| 3. | (b) and (d) only | 4. | (b) and (c) only |
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
| 1. | ELISA Technique | 2. | Hybridization Technique |
| 3. | Western Blotting Technique | 4. | Southern Blotting Technique |
The Adenosine deaminase deficiency results into:
| 1. | Digestive disorder | 2. | Addison's disease |
| 3. | Dysfunction of Immune system | 4. | Parkinson's disease |
Match the following columns and select the correct option.
| Column-I | Column-II | ||
| a. | Bt cotton | (i) | Gene therapy |
| b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
| c. | RNAi | (iii) | Detection of HIV infection |
| d. | PCR | (iv) | Bacillus thuringiensis |
| a | b | c | d | |
| 1. | (iii) | (ii) | (i) | (iv) |
| 2. | (ii) | (iii) | (iv) | (i) |
| 3. | (i) | (ii) | (iii) | (iv) |
| 4. | (iv) | (i) | (ii) | (iii) |
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
| (a) | (b) | (c) | (d) | |
| 1. | (iv) | (iii) | (i) | (ii) |
| 2. | (iii) | (ii) | (iv) | (i) |
| 3. | (iii) | (iv) | (i) | (ii) |
| 4. | (ii) | (iv) | (iii) | (i) |
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