Match the following techniques or instruments with their usage:

(a)	Bioreactor	(i)	Separation of DNA fragments
(b)	Electrophoresis	(ii)	Production of large quantities of products
(c)	PCR	(iii)	Detection of pathogen, based on antigen-antibody reaction
(d)	ELISA	(iv)	Amplification of nucleic acids

Select the correct option from the following:

1.	(a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2.	(a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
3.	(a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)
4.	(a)-(ii), (b)-(i), (c)-(iii), (d)-(iv)

In a mixture, DNA fragments are separated by-

1. Bioprocess engineering

2. Restriction digestion

3. Electrophoresis

4. Polymerase chain reaction

Spooling is:

Select the correct statement from the following:

In genetic engineering, the antibiotic-resistance gene is used:
1.  To select healthy vectors
2.  As sequences from where replication starts
3.  To keep the cultures free of infection
4.  As selectable markers

Which of the following are used in gene cloning:

1. Lomasomes

2. Mesosomes

3. Plasmids

4. Nucleoids

In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:

Options:

Which one of the following techniques made it possible to genetically engineer living organisms?

1. Hybridization

2. Recombinant DNA techniques

3. X-ray diffraction

4. Heavier isotope labelling

First discovered restriction endonuclease that always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs is:

1. EcoRI

2. Adenosine deaminase

3. Thermostable DNA polymerase

4. Hind II

Genetic engineering involves:
1. Use of restriction endonuclease on bacterial DNA and formation of new traits
2. Use of Ligase for cutting DNA
3. Developing instruments
4. Use of statistic in genetics