Separation and purification by filtration, centrifugation of desired compound produced in bioreactor is a part of

1. Downstream processing only

2. Scaling up and downstream processing

3. Upstream processing

4. Screening for recombinants and downstream processing

Which of the following is not a method of introducing alien DNA into host cells?

1. Microinjection

2. Heat shock method

3. Being placed along with the cell into a gene gun

4. Gel electrophoresis

Mark the correct statement for pBR322 as a plasmid
vector
I. Contains relaxed origin of replication.
II. Two genes coding for resistance to antibiotics have
been introduced.
III. There are single recognition sites for a number of
restriction enzymes at various points.
IV. Insertional inactivation is a useful selection method
for identifying recombinant vectors with insert.

1. I, II, III & IV 

2. I, II only

3. II, III only 

4. II only

How does a bacterial cell protect its own DNA from
restriction enzymes?

1. By adding methyl groups to adenine and
cytosines.
2. By reinforcing bacterial DNA structure with covalent
phosphodiester bonds.
3. By adding histones to protect the double-stranded
DNA.
4. By forming “sticky ends” of bacterial DNA to prevent
the enzyme from attaching

DNA fragments separated by gel electrophoresis are shown. Mark the correct statement :

Which of the following is not a direct method of gene transfer?

1. Microinjection
2. Biolistic
3. Electroporation
4. Disarmed pathogen vector

The restriction endonuclease breaks bonds between:

1. DNA-RNA hybrid

2. Introns

3. Nucleotides, i.e., breaks the phosphodiester bond

4. Pentose sugar and nitrogenous base, i.e., breaks N-glycosidic bond

During gel electrophoresis for separation of DNA fragment:

1. Smallest fragment will move to the farthest point towards cathode

2. Smallest fragment will move to the farthest point towards anode

3. Largest fragment will move to the farthest point towards cathode

4. Largest fragment will move to the farthesr point towards anode

When we ligate a foreign DNA at the sal I site of tetracycline resistance gene in a vector pBR 322, the ____a____ plasmid will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from ____b____ ones by plating the ___c____ on ampicillin containing medium

        a                                b                                   c

1. Non-recombinant     Recombinant                 Transformant

2. Recombinant           Non-recombinant            Transformant

3. Transformant           Non-recombinant            Recombinant

4. Recombinant            Transformant                  Non-recombinant

If Pvu I restriction endonuclease is used for cleaving pBR322 cloning vector for inserting foreign DNA, which of the following can be expected in recombinants?

1. They remain resistant to tetracycline but not to ampicillin

2. They would die in tetracycline containing medium but remain alive in ampicillin containing medium

3. Replication of recombinant plasmid get prevented

4. They do not show resistance to ampicillin as well as tetracycline