Q. No.Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards :
| 1. | Gentamycin |
| 2. | Ampicillin and tetracycline |
| 3. | Tetracycline |
| 4. | Ampicillin |
Subtopic: Cloning Vector |
77%
Level 2: 60%+
NEET - 2026
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Match List-I with List-II:
Choose the correct answer from the options given below :
1. A-IV, B-I, C-III, D-II
2. A-II, B-I, C-IV, D-III
3. A-I, B-II, C-IV, D-III
4. A-III, B-IV, C-II, D-I
| List-I | List-II | ||
| A. | Transformation | I. | Restriction enzyme |
| B. | Cloning site | II. | Transfer DNA to host bacteria |
| C. | Selection | III. | Replication |
| D. | Ori | IV. | Antibiotic |
Choose the correct answer from the options given below :
1. A-IV, B-I, C-III, D-II
2. A-II, B-I, C-IV, D-III
3. A-I, B-II, C-IV, D-III
4. A-III, B-IV, C-II, D-I
Subtopic: Cloning Vector |
93%
Level 1: 80%+
NEET - 2026
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Given below are two statements:
In the light of the above statements, choose the most appropriate answer from the options given below:
| Statement I: | Plasmids are autonomously replicating DNA. |
| Statement II: | Plasmids are extrachromosomal DNA. |
In the light of the above statements, choose the most appropriate answer from the options given below:
| 1. | Statement I is incorrect but Statement II is correct |
| 2. | Both Statement I and Statement II are correct |
| 3. | Both Statement I and Statement II are incorrect |
| 4. | Statement I is correct but Statement II is incorrect |
Subtopic: Cloning Vector | Principles of Biotechnology |
92%
Level 1: 80%+
NEET - 2026
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In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Subtopic: Cloning Vector |
62%
Level 2: 60%+
NEET - 2025
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
76%
Level 2: 60%+
NEET - 2024
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
89%
Level 1: 80%+
NEET - 2024
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| A. | Ampicillin | I. | Restriction sites |
| B. | Ori | II. | Blue/White screening |
| C. | Multiple cloning sites | III. | Selectable marker |
| D. | \(\beta\) galactosidase gene | IV. | Sequence from where replication starts |
| 1. | A-III, B-IV, C-I , D-II | 2. | A-III, B-I, C-IV, D-II |
| 3. | A-IV, B-III, C-II , D-I | 4. | A-II, B-IV, C-I, D-III |
Subtopic: Cloning Vector |
85%
Level 1: 80%+
NEET - 2024
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
87%
Level 1: 80%+
NEET - 2024
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
Subtopic: Cloning Vector |
86%
Level 1: 80%+
NEET - 2023
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
54%
Level 3: 35%-60%
NEET - 2023
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