Q. No.Which of the following statements are correct with respect to DNA separation, isolation and visualization ?
Choose the correct answer from the options given below:
1. A and D only
2. B and D only
3. B and C only
4. A and B only
| A. | The cutting of DNA is done by molecular scissors. |
| B. | The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. |
| C. | The separated DNA fragments can be seen without staining when exposed to UV light. |
| D. | The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. |
Choose the correct answer from the options given below:
1. A and D only
2. B and D only
3. B and C only
4. A and B only
Subtopic: Â Separation and Isolation of DNA fragments |
 72%
Level 2: 60%+
NEET - 2026
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the most appropriate answer from the options given below:
| Assertion (A): | In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells. |
| Reason (R): | Isolation of genetic material needs disruption of cells. |
In the light of the above statements, choose the most appropriate answer from the options given below:
| 1. | (A) is not correct but (R) is correct |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A) |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A) |
| 4. | (A) is correct but (R) is not correct |
Subtopic: Â Separation and Isolation of DNA fragments |
 69%
Level 2: 60%+
NEET - 2026
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Identify the incorrect statement related to electrophoresis:
| 1. | Separated DNA fragments can be directly seen under UV radiation |
| 2. | Separated DNA can be extracted from gel piece. |
| 3. | Fragments of DNA move toward anode. |
| 4. | Sieving effect of agarose gel helps in separation of DNA fragments. |
Subtopic: Â Separation and Isolation of DNA fragments |
 79%
Level 2: 60%+
NEET - 2024
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Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
| 1. | Bright orange colour | 2. | Bright red colour |
| 3. | Bright Blue colour | 4. | Bright yellow colour |
Subtopic: Â Separation and Isolation of DNA fragments |
 93%
Level 1: 80%+
NEET - 2023
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Which one of the following statement is not true regarding gel electrophoresis technique?
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
Subtopic: Â Separation and Isolation of DNA fragments |
 84%
Level 1: 80%+
NEET - 2022
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Â Separation and Isolation of DNA fragments |
 90%
Level 1: 80%+
NEET - 2022
Hints
Refer to the following statements for agarose-gel electrophoresis:
Choose the most appropriate answer from the options given below :
| (a) | Agarose is a natural polymer obtained from sea-weed. |
| (b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
| (c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
| (d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
| 1. | (a) and (b) only | 2. | (a), (b) and (c) only |
| 3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
Subtopic: Â Separation and Isolation of DNA fragments |
 83%
Level 1: 80%+
NEET - 2022
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DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
Subtopic: Â Separation and Isolation of DNA fragments |
 93%
Level 1: 80%+
NEET - 2021
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Histones | 2. | Polysaccharides |
| 3. | RNA | 4. | DNA |
Subtopic: Â Separation and Isolation of DNA fragments |Â General Design of an rDNA experiment |
 90%
Level 1: 80%+
NEET - 2021
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In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Subtopic: Â Separation and Isolation of DNA fragments |
 94%
Level 1: 80%+
NEET - 2020
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