- 1(current)
Q. No.Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: Â General Design of an rDNA experiment |
 79%
Level 2: 60%+
Hints
Match List I with List II:
Choose the correct answer from the options given below:
1. A - (III); B - (IV); C- (II); D - (I)
2. A - (III); B - (IV); C- (I); D - (II)
3. A - (IV); B - (III); C- (II); D - (I)
4. A - (IV); B - (III); C- (I); D - (II)
| List I | List II |
| A. Chloramphenicol | I. Join the cut ends of DNA molecule |
| B. Ethidium bromide | II. Makes bacterial cell competent to take up DNA |
| C. DNA ligase | III. Selectable marker |
| D. Calcium | IV. Agarose gel staining |
1. A - (III); B - (IV); C- (II); D - (I)
2. A - (III); B - (IV); C- (I); D - (II)
3. A - (IV); B - (III); C- (II); D - (I)
4. A - (IV); B - (III); C- (I); D - (II)
Subtopic: Â General Design of an rDNA experiment |
 92%
Level 1: 80%+
Hints
- 1(current)
Q. No.
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