Given below are the three basic steps in genetically modifying an organism
| a. | Introduction of the identified DNA into the host. |
| b. | Maintenance of introduced DNA in the host and transfer of the DNA to its progeny. |
| c. | Identification of DNA with desirable genes. |
Choose the option with respect to correct sequence of the given steps
1. a → c → b
2. c → b → a
3. c → a → b
4. b → a → c
Following enzymes/techniques are used in the process of recombinant DNA technology
| a. | EcoRl to cut the isolated genome |
| b. | DNA ligase |
| c. | Protease and ribonuclease for removal of proteins and RNA from DNA |
| d. | Production of recombinant hosts |
| e. | Lysozyme for isolation of the genetic material (DNA) |
| f. | Gel electrophoresis for separation and isolation of DNA fragments |
Mark the correct sequence of their use
1. c, e, b, f, a, d
2. e, c, a, b, f, d
3. e, c, a, f, b, d
4. a, e, c, b, d, f
What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
| I: | At A, same restriction enzyme is used to cut both foreign and vector DNA |
| II: | The enzyme used at B is DNA ligase |
| III: | Step C can be called as transformation |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |