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Q. No.
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Assertion(A):
If BamHI cuts a 12 kb linear dsDNA fragment at two restriction sites which are at the same distance from the ends and each other, then only one band is obtained on agarose gel electrophoresis.
Reason(R):
Three equal sized fragments will be obtained on restriction digestion by BamHI.
1.
Both (A) and (R) are True and (R) is the correct explanation of (A).
2.
Both (A) and (R) are True but (R) is not the correct explanation of (A).
3.
(A) is false but (R) is true.
4.
(A) is true but (R) is false
Subtopic: Tools: Enzymes: I |
Level 3: 35%-60%
Hints
In agarose gel electrophoresis, DNA molecules are separated on the basis of
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
Subtopic: Separation and Isolation of DNA fragments |
81%
Level 1: 80%+
Hints
Match Column-I with Column-II and select the correct option:
1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
| Column-I | Column-II | ||
| a. | Restriction endonuclease | (i) | Hind II |
| b. | First recombinant DNA | (ii) | Obtained from strain RY13 |
| c. | First restriction endonuclease | (iii) | Isolated in 1963 |
| d. | EcoRI | (iv) | Developed in 1972 |
1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic: Restriction Enzymes: Historical Background | Introduction & History |
83%
Level 1: 80%+
Hints
Read the following statements and choose the correct option.
1. Only statement A is correct
2. Only statement A is incorrect
3. Both A and B statements are correct
4. Both A and B statements are incorrect
| Statement (A): | DNA cannot pass through cell membranes as it is a hydrophobic molecule. |
| Statement (B): | Treatment with Ca2+ ions increases the efficiency with which DNA enters into the bacterium. |
2. Only statement A is incorrect
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Transforming Plant & Animal Cell |
64%
Level 2: 60%+
Hints
Match column-I with column-II and choose the correct option:
1. a(iv), b(ii), c(iii), d(i)
2. a(iv), b(ii), c(i), d(iii)
3. a(ii), b(iv), c(i), d(iii)
4. a(ii), b(iv), c(iii), d(i)
| Column-I | Column -II | ||
| a. | Biosynthetic stage | (i) | Increased surface area for oxygen transfer |
| b. | Downstream processing | (ii) | Large-scale production in the fermenter |
| c. | Sparged stirred-tank bioreactor |
(iii) | With foam breaker and flat bladed impeller |
| d. | Simple stirred-tank bioreactor |
(iv) | Separation and purification |
1. a(iv), b(ii), c(iii), d(i)
2. a(iv), b(ii), c(i), d(iii)
3. a(ii), b(iv), c(i), d(iii)
4. a(ii), b(iv), c(iii), d(i)
Subtopic: Bioreactors/Downstream Processing | Large Scale Production |
73%
Level 2: 60%+
Hints
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Read the following statements and choose the correct option
Statement A: CaCl2 treatment increases the efficiency of DNA uptake to produce transformed bacterial cells
Statement B: The divalent Ca+2 ions create transient pores in the bacteria
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Statement A: CaCl2 treatment increases the efficiency of DNA uptake to produce transformed bacterial cells
Statement B: The divalent Ca+2 ions create transient pores in the bacteria
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Transforming Plant & Animal Cell |
Level 4: Below 35%
Hints
Read the following statements and choose the correct option
| Statement A: | Wine making as well as antigen production in recombinant organisms are included in biotechnology |
| Statement B: | Biotechnology is a very wide area which deals with techniques of using a natural organism (or its parts) as well as genetic engineering |
| 1. | Only statement A is correct |
| 2. | Only statement B is correct |
| 3. | Both statements A and B are correct |
| 4. | Both statements A and B are incorrect |
Subtopic: Introduction & History: I |
80%
Level 1: 80%+
Hints
If restriction endonuclease cuts the DNA a little away from the centre of the palindromic site, then what would happen?
Choose the correct option
1. (a) and (b) are correct
2. (a) and (c) are correct
3. (b) and (c) are correct
4. (a), (b) and (c) are correct
| (a) | The DNA fragments obtained will have 'sticky ends' |
| (b) | Construction of rDNA molecule would not be possible |
| (c) | Resultant DNA fragments with overhanging streches can be ligated with the help of DNA ligase |
Choose the correct option
1. (a) and (b) are correct
2. (a) and (c) are correct
3. (b) and (c) are correct
4. (a), (b) and (c) are correct
Subtopic: Restriction Enzymes - Main Enzymes |
85%
Level 1: 80%+
Hints
How many of the following features are correct with respect to plasmids?
1. Zero
2. Two
3. Three
4. One
| (a) | Extrachromosomal DNA molecule |
| (b) | Replicate independent of chromosomal DNA |
| (c) | Widely used as cloning vectors for animals host cells only |
2. Two
3. Three
4. One
Subtopic: Tools: Vectors: I | Tools: Vectors: II |
76%
Level 2: 60%+
Hints
Read the following statements and choose the correct option:
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
| Statement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
| Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
Subtopic: Cloning Vector |
73%
Level 2: 60%+
Hints
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