In bacteria, the plasmid is:

1. Extrachromosomal material

2. Main DNA

3. Non-functional DNA

4. Repetitive gene

Which of the following enzymes are used to join bits of DNA:

1. Ligase

2. Primase

3. DNA polymerase

4. Endonuclease

Manipulation of DNA in genetic engineering became possible due to the discovery of:

1. Restriction endonuclease

2. DNA ligase

3. Transcriptase

4. Primase

Which one of the following bacteria has found extensive use in genetic engineering work in plants?
1. Clostridium septicum
2. Xanthomonas citri
3. Bacillus coagulens
4. Agrobacterium tumefaciens

What is true for a plasmid?
1. Plasmids are widely used in gene transfer
2. These are found in a virus
3. A plasmid contains genes for vital activities
4. This is the main part of the chromosome

Which of the following cut the DNA from specific places:
1.  Restriction endonuclease (EcoRI)
2.  Ligase
3.  Exonuclease
4.  Alkaline phosphate

Maximum number of bases in plasmids discovered so far:
1.  50 kilobase
2.  500 kilobase
3.  5000 kilobase
4.  5 kilobase

In Lederberg's replica plating experiment, what shall be used to obtain streptomycin resistant strain?
1.  Minimal medium and streptomycin
2.  Complete medium and streptomycin
3.  Only minimal medium
4.  Only complete medium

Plasmid has been used as a vector because:
 

A giant rat is formed in the laboratory, what is the reason:

1. Gene mutation

2. Gene synthesis

3. Gene manipulation

4. Gene replication