- 1(current)
Q. No.Which of the following can act as selectable markers in a cloning vector?
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
Subtopic: Â Cloning Vector |
 86%
From NCERT
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Read the following statements and choose the correct option:
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
| Statement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
| Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
Subtopic: Â Cloning Vector |
 72%
From NCERT
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Assertion(A): Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Cloning Vector |Â Introduction & History |
 61%
From NCERT
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Assertion(A): In order to link the alien DNA, the vector needs to have very few, preferably single, recognition site for the commoniy used restriction enzymes.
Reason(R): Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â Cloning Vector |
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From NCERT
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Assertion(A): Plasmids are extrachromosomal gentical material of bacteria.
Reason(R): These are genetic material present apart from the nucleoid.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): These are genetic material present apart from the nucleoid.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â Cloning Vector |
 66%
From NCERT
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Assertion(A): Plasmids as cloning vectors usually get integrated to the host cells chromosome after transformation.
Reason(R): Plasmids get disintegrated after transformation.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Plasmids get disintegrated after transformation.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â Cloning Vector |
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Read the following statements:
(i) Restriction endonucleases are obtained only from prokaryotes
(ii) Sma I creats blunt ends in plasmids
(iii) Blunt ends can be made sticky with the help of enzyme phosphatase
(iv) Size of pBR322 is 43 kb
(v) Shuttle vectors possess two types of ori-sites How many of the above statements are incorrect?
1. Three
2. Two
3. One
4. Four
(i) Restriction endonucleases are obtained only from prokaryotes
(ii) Sma I creats blunt ends in plasmids
(iii) Blunt ends can be made sticky with the help of enzyme phosphatase
(iv) Size of pBR322 is 43 kb
(v) Shuttle vectors possess two types of ori-sites How many of the above statements are incorrect?
1. Three
2. Two
3. One
4. Four
Subtopic: Â Tools: Vectors: I |Â Cloning Vector |Â Tools |
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Â Cloning Vector |Â Tools |
 86%
From NCERT
NEET - 2022
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NEET 2023 - Target Batch - Aryan Raj Singh
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NEET 2023 - Target Batch - Aryan Raj Singh
- 1(current)
Q. No.
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