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Q. No.Statement I: Ethidium bromide-stained DNA can be seen under exposure to UV light when DNA molecules are separated by agarose gel electrophoresis.
Statement II: Ethidium bromide-stained DNA can be cut out and extracted from the agarose gel piece.
In the light of the above statements, choose the most appropriate answer from the options given below:
1. Both Statement I and Statement II are correct.
2. Both Statement I and Statement II are incorrect.
3. Statement I is correct but Statement II is incorrect.
4. Statement I is incorrect but Statement II is correct.
Subtopic: Separation and Isolation of DNA fragments |
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Assertion(A): In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason(R): Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Tools: Vectors: I | Host & Desired DNA: I | Host & Desired DNA |
76%
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Assertion(A): Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Restriction Enzymes - Main Enzymes | Cloning Vector | Introduction & History |
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Assertion(A): Chitinase is used for treating the cells of yeast to assist DNA extraction.
Reason(R): Yeast cell wall is made of chitin.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Yeast cell wall is made of chitin.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Separation and Isolation of DNA fragments |
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Assertion(A): Restriction enzymes are molecular scissors.
Reason(R): They identify DNA sequence and cut them.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): They identify DNA sequence and cut them.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Selectable Markers |
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Assertion(A): The first discovered restriction endonuclease was Hind II.
Reason(R): Hind III was discovered earlier than Hind II.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Hind III was discovered earlier than Hind II.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Tools: Vectors: I | Selectable Markers: I | Selectable Markers: II |
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Assertion(A): Palindromic sequences are identified by restriction endonucleases.
Reason(R): Endonucleases cannot destroy the phosphodiester bonds other than those of palindromes.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Endonucleases cannot destroy the phosphodiester bonds other than those of palindromes.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Selectable Markers: I | Selectable Markers: II |
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Assertion(A): While loading the DNA fragment into the wells of agarose gel, a blue dye methylene blue is mixed.
Reason(R): Radioactive methylene blue is used.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Radioactive methylene blue is used.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Separation and Isolation of DNA fragments |
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Assertion(A): In order to link the alien DNA, the vector needs to have very few, preferably single, recognition site for the commoniy used restriction enzymes.
Reason(R): Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Cloning Vector |
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Assertion(A): Only antibiotic resistant gene in vectors can be used as selectable markers.
Reason(R): p-gal gene cannot be used in plasmid vectors as selectable markers.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): p-gal gene cannot be used in plasmid vectors as selectable markers.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Selection of Recombinant Transformants |
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