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Q. No.
Assertion (A):
In vitro fertilisation, leading to a 'test-tube' baby, is included under the umbrella of biotechnology
Reason (R):
The definition of biotechnology, given by EFB encompasses only modern molecular biotechnology view
Choose the correct answer from the options given below:
1.
(A) is true but (R) is false
2.
(A) is false but (R) is true
3.
Both (A) and (R) are true and (R) is the correct explanation of (A)
4.
Both (A) and (R) are true but (R) is the not the correct explanation of (A)
Subtopic: Introduction & History |
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Read the following statements and choose the correct option.
1. Both statements are incorrect
2. Only statement A is correct
3. Only statement B is correct
4. Both statements are correct
| Statement A: | Plasmid is a double stranded structure. |
| Statement B: | Plasmid is extra chromosomal genetic material in bacteria. |
2. Only statement A is correct
3. Only statement B is correct
4. Both statements are correct
Subtopic: Host & Desired DNA: I | Host & Desired DNA: II |
81%
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Consider the given statements and select the option that correctly states them as true (T) or false (F).
(a) (b) (c)
1. T T T
2. T F T
3. T F F
4. F F T
| (a) | A gene whose expression helps to identify transformed cell is known as a selectable marker. |
| (b) | BOD incubator is essentially required for growing microbes on a large scale, for industrial production of enzymes. |
| (c) | For transformation, micro-particles coated with DNA to be bombarbed using gene gun are made up of gold or tungsten. |
1. T T T
2. T F T
3. T F F
4. F F T
Subtopic: Selection of Recombinant Transformants |
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Consider the given statements and select the option including incorrect statements only.
(a) Continuous addition of sugars in ‘fed batch’ fermentation is done to purify enzymes.
(b) Heavier isotope labelling made possible to genetically engineer the living organisms.
(c) Production of a human protein in bacteria by genetic engineering is possible because the genetic code is universal.
1. (a) and (b)
2. (a) and (c)
3. (b) and (c)
4. (a), (b) and (c)
(a) Continuous addition of sugars in ‘fed batch’ fermentation is done to purify enzymes.
(b) Heavier isotope labelling made possible to genetically engineer the living organisms.
(c) Production of a human protein in bacteria by genetic engineering is possible because the genetic code is universal.
1. (a) and (b)
2. (a) and (c)
3. (b) and (c)
4. (a), (b) and (c)
Subtopic: Process of Biotech |
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| Assertion: | E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. |
| Reason: | Recognition site for Bam HI is present in tetR region of pBR322. |
| 1. | Both assertion and reason are true, and the reason is the correct explanation of the assertion. |
| 2. | Both assertion and reason are true, but the reason is not the correct explanation of the assertion. |
| 3. | Assertion is true but reason is false. |
| 4. | Both assertion and reason are false. |
Subtopic: Selection of Recombinant Transformants |
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Statement I: Ethidium bromide-stained DNA can be seen under exposure to UV light when DNA molecules are separated by agarose gel electrophoresis.
Statement II: Ethidium bromide-stained DNA can be cut out and extracted from the agarose gel piece.
In the light of the above statements, choose the most appropriate answer from the options given below:
1. Both Statement I and Statement II are correct.
2. Both Statement I and Statement II are incorrect.
3. Statement I is correct but Statement II is incorrect.
4. Statement I is incorrect but Statement II is correct.
Statement II: Ethidium bromide-stained DNA can be cut out and extracted from the agarose gel piece.
In the light of the above statements, choose the most appropriate answer from the options given below:
1. Both Statement I and Statement II are correct.
2. Both Statement I and Statement II are incorrect.
3. Statement I is correct but Statement II is incorrect.
4. Statement I is incorrect but Statement II is correct.
Subtopic: Separation and Isolation of DNA fragments |
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Assertion(A): In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason(R): Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Tools: Vectors: I | Host & Desired DNA: I | Host & Desired DNA |
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Assertion(A): Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Restriction Enzymes - Main Enzymes | Cloning Vector | Introduction & History |
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Assertion(A): Chitinase is used for treating the cells of yeast to assist DNA extraction.
Reason(R): Yeast cell wall is made of chitin.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Yeast cell wall is made of chitin.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Separation and Isolation of DNA fragments |
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Assertion(A): Restriction enzymes are molecular scissors.
Reason(R): They identify DNA sequence and cut them.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): They identify DNA sequence and cut them.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Selectable Markers |
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