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Q. No.How many of the following features are correct with respect to plasmids?
(a)
Extrachromosomal DNA molecule
(b)
Replicate independent of chromosomal DNA
(c)
Widely used as cloning vectors for animals host cells only
1. Zero
2. Two
3. Three
4. One
Subtopic: Tools: Vectors: I | Tools: Vectors: II |
75%
Level 2: 60%+
Hints
Read the following statements and choose the correct option:
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
| Statement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
| Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
Subtopic: Cloning Vector |
73%
Level 2: 60%+
Hints
Match the dyes listed in column I with their usage listed in column II and choose the correct option
1. a(iv), b(iii), c(i), d(ii)
2. a(ii), b(iv), c(i), d(iii)
3. a(iv), b(i), c(ii), d(iii)
4. a(iii), b(ii), c(i), d(iv)
| Column I | Column II | ||
| a. | Aniline blue | (i) | Used to stain DNA in agarose gel electrophoresis |
| b. | Bromophenol blue | (ii) | stains chromosomes |
| c. | Ethidium bromide | (iii) | Tracking dye |
| d. | Acetocarmine | (iv) | Used to stain collagen |
1. a(iv), b(iii), c(i), d(ii)
2. a(ii), b(iv), c(i), d(iii)
3. a(iv), b(i), c(ii), d(iii)
4. a(iii), b(ii), c(i), d(iv)
Subtopic: Tools |
57%
Level 3: 35%-60%
Hints
Read the following statements and choose the correct option:
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
| Statement A: | Agarose gel electrophoresis is employed to separate the fragments of DNA obtained after restriction enzyme digestion. |
| Statement B: | DNA is a negatively charged molecule and moves toward the cathode. |
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Separation and Isolation of DNA fragments |
80%
Level 1: 80%+
Hints
| Assertion (A): | In vitro fertilisation, leading to a 'test-tube' baby, is included under the umbrella of biotechnology |
| Reason (R): | The definition of biotechnology, given by EFB encompasses only modern molecular biotechnology view |
Choose the correct answer from the options given below:
| 1. | (A) is true but (R) is false |
| 2. | (A) is false but (R) is true |
| 3. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 4. | Both (A) and (R) are true but (R) is the not the correct explanation of (A) |
Subtopic: Introduction & History |
78%
Level 2: 60%+
Hints
Read the following statements and choose the correct option.
| Statement A: | Plasmid is a double stranded structure. |
| Statement B: | Plasmid is extra chromosomal genetic material in bacteria. |
| 1. | Both statements are incorrect |
| 2. | Only statement A is correct |
| 3. | Only statement B is correct |
| 4. | Both statements are correct |
Subtopic: Host & Desired DNA: I | Host & Desired DNA: II |
83%
Level 1: 80%+
Hints
Consider the given statements and select the option that correctly states them as true (T) or false (F).
| (a) | A gene whose expression helps to identify transformed cell is known as a selectable marker. |
| (b) | BOD incubator is essentially required for growing microbes on a large scale, for industrial production of enzymes. |
| (c) | For transformation, micro-particles coated with DNA to be bombarbed using gene gun are made up of gold or tungsten. |
| (a) | (b) | (c) | |
| 1. | T | T | T |
| 2. | T | F | T |
| 3. | T | F | F |
| 4. | F | F | T |
Subtopic: Selection of Recombinant Transformants |
Level 3: 35%-60%
Hints
Consider the given statements and select the option including incorrect statements only.
(a) Continuous addition of sugars in ‘fed batch’ fermentation is done to purify enzymes.
(b) Heavier isotope labelling made possible to genetically engineer the living organisms.
(c) Production of a human protein in bacteria by genetic engineering is possible because the genetic code is universal.
1. (a) and (b)
2. (a) and (c)
3. (b) and (c)
4. (a), (b) and (c)
(a) Continuous addition of sugars in ‘fed batch’ fermentation is done to purify enzymes.
(b) Heavier isotope labelling made possible to genetically engineer the living organisms.
(c) Production of a human protein in bacteria by genetic engineering is possible because the genetic code is universal.
1. (a) and (b)
2. (a) and (c)
3. (b) and (c)
4. (a), (b) and (c)
Subtopic: Process of Biotech |
53%
Level 3: 35%-60%
Hints
| Assertion: | E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. |
| Reason: | Recognition site for Bam HI is present in tetR region of pBR322. |
| 1. | Both assertion and reason are true, and the reason is the correct explanation of the assertion. |
| 2. | Both assertion and reason are true, but the reason is not the correct explanation of the assertion. |
| 3. | Assertion is true but reason is false. |
| 4. | Both assertion and reason are false. |
Subtopic: Selection of Recombinant Transformants |
Level 4: Below 35%
Hints
| Statement I: | Ethidium bromide-stained DNA can be seen under exposure to UV light when DNA molecules are separated by agarose gel electrophoresis. |
| Statement II: | Ethidium bromide-stained DNA can be cut out and extracted from the agarose gel piece. |
In the light of the above statements, choose the most appropriate answer from the options given below:
| 1. | Both Statement I and Statement II are correct. |
| 2. | Both Statement I and Statement II are incorrect. |
| 3. | Statement I is correct but Statement II is incorrect. |
| 4. | Statement I is incorrect but Statement II is correct. |
Subtopic: Separation and Isolation of DNA fragments |
89%
Level 1: 80%+
Hints
Select Chapter Topics:
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