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The colonies of recombinant bacteria appear white because of:
1.
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
2.
Insertional inactivation of beta-galactosidase in recombinant bacteria
3.
Inactivation of glycosidase enzyme in recombinant bacteria
4.
Non-recombinant bacteria containing beta-galactosidase
Subtopic: Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
81%
Level 1: 80%+
AIPMT - 2013
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The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes
Subtopic: Tools: Vectors: II | Cloning Vector | Tools |
87%
Level 1: 80%+
AIPMT - 2012
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Which one of the following is a case of wrong matching?
| 1. | Somatic hybridization- Fusion of two diverse cells |
| 2. | Vector DNA- Site for tRNA synthesis |
| 3. | Micropropagation- In vitro production of plants in large numbers |
| 4. | Callus - Unorganized mass of cells produced in tissue culture |
Subtopic: Tools: Vectors: I | Tools |
77%
Level 2: 60%+
AIPMT - 2012
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Which one is a true statement regarding DNA polymerase used in PCR?
| 1. | It is used to ligate introduces DNA in recipient cells |
| 2. | It serves as a selectable marker |
| 3. | It is isolated from a virus |
| 4. | It remains active at high temperatures |
Subtopic: Polymerase Chain Reaction: PCR |
78%
Level 2: 60%+
AIPMT - 2012
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For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:
1. Silver or platinum
2. Platinum or zinc
3. Silicon or platinum
4. Gold or tungsten
Subtopic: Transforming Plant & Animal Cell |
96%
Level 1: 80%+
AIPMT - 2012
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Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it?
5’___GAATTC___3’
3’___CTTAAG__5’
5’___GAATTC___3’
3’___CTTAAG__5’
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
Subtopic: Tools: Enzymes: II | Restriction Enzymes - Main Enzymes | Tools |
96%
Level 1: 80%+
AIPMT - 2011
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There is a restriction endonuclease called Eco RI. What does the 'co' part in it stand for?
1. Coelom
2. Coenzyme
3. Coli
4. Colon
1. Coelom
2. Coenzyme
3. Coli
4. Colon
Subtopic: Restriction Enzymes - Main Enzymes |
90%
Level 1: 80%+
AIPMT - 2011
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Agarose extracted from seaweeds finds use in:
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
Subtopic: Separation and Isolation of DNA fragments |
95%
Level 1: 80%+
AIPMT - 2011
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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
| 1. | 5'-CGTTCG-3' | 2. | 5'-GATATG-3' |
| 3'-ATGGTA-5' | 3'-CTACTA-5' | ||
| 3. | 5'-GAATTC-3' | 4. | 5'-CACGTA-3' |
| 3'-CTTAAG-5' | 3'-CTCAGT-5' |
Subtopic: Restriction Enzymes - Main Enzymes |
94%
Level 1: 80%+
AIPMT - 2010
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Restriction endonucleases are enzymes which:
| 1. | make cuts at specific positions within the DNA molecule |
| 2. | recognize a specific nucleotide sequence for binding of DNA ligase |
| 3. | restrict the action of the enzyme DNA polymerase |
| 4. | remove nucleotides from the ends of the DNA molecule |
Subtopic: Restriction Enzymes - Main Enzymes |
92%
Level 1: 80%+
AIPMT - 2010
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