Given below is the diagram of agarose gel kept under UV light:
(a) Mention the positive and negative terminals .
(b) What is the charge carried by DNA molecule and how does it help in its separation?
(c) How are the separted DNA fragments finally isolated?
(a) Positive terminal-'B'
(b) DNA molecule carries a negative charge. DNA being negatively charged, moves towards the positive electrode (anode).
(c) The separated DNA fragments are isolated by elution. The seperated bands of DNA are cut out from the agarose gel and are extracted from the gel piece.