After 30 cycle of PCR, how many times DNA will be amplified?

A.      Thousand times

B.      Million times

C.      Billion times

D.     Hundreds times

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Use of primers, occurs in which step of PCR?

A.      Annealing

B.      Extension

C.      Denaturation

D.     Both extension and Annealing

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If you have transformed cell with recombinant DNA and it grows on kanamycin and chloramphenicol containing plates but does not grow on ampicillin containing medium. What does it imply?

A.      The cell has resistance against ampicillin but not against chloramphenicol and kanamycin

B.      Has resistance against both kanamycin and chloramphenicol but not against ampicillin

C.      We may not confer anything from it

D.     It depends upon gene of interest

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The ultimate aim of maximum recombinant DNA technologies is

A.    To multiply DNA

B.    To express DNA

C.   To multiply mRNA

D.   To express protein

Concept Questions :-

Large Scale Production:III
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Which of the following processes will be last?

A.      Purification of DNA

B.      Purification of protein

C.      Expression of protein

D.     Multiplication of recombinant DNA

Concept Questions :-

Large Scale Production: II
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Which of the following processes is not associated with protein produced by recombinant DNA?

A.      Restriction Digestion

B.      Expression

C.      Optimization

D.     Purification

Concept Questions :-

Tools: Enzymes:II
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Which of the following processes is done at the level of DNA only?

A.      Optimization

B.      Expression

C.      Purification

D.     Restriction Digestion

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What does continuous culture mean?

A.      Where DNA and protein getting expressed continuously

B.      Where used medium is drained out and fresh medium is  added from other side

C.      Where cells are producing protein for one week continuously

D.     Where production of recombinant DNA is continuing without interference.

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Continuous culture has

A.      Cells in their physically active phase

B.      Lag phase

C.      Both A and B

D.     Exponential phase

Concept Questions :-

Large Scale Production: II
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Continuous culture leads to

A.      Smaller biomass

B.      Higher yields of desired proteins

C.      Both A and B

D.     Higher yields of recombinant DNA

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