The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

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For the synthesis of 4 copies of DNA, how many sets of primers are needed in PCR

A.      2 sets

B.      8 sets

C.      4 sets

D.     Random

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The synthesis of DNA in PCR will be in

A.      5’-3’

B.      3’-5’

C.      It won't be depending on direction

D.     For one strand 5’-3’ in another opposite to it

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The primers designed according to (Answer according to very basic PCR)

A.      Terminal ends of vector

B.      Terminal ends of gene of interest

C.      Random primers

D.     According to direction of PCR

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Primers are not

A.      Small

B.      Biologically synthesised

C.      Oligonucleotides

D.     Complementary to the regions of DNA

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Which of the following doesn't go with PCR?

A.      Thermus aquaticus

B.      1 billion copies

C.      Taq polymerase

D.     Dideoxynucleotides

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If a strand has following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.

A.      5’TAACGGGATC 3’

B.      3’TAACGGGATC 5’

C.      5’ATTGCCCTAG 3’

D.     3’ATTGCCCTAG 5’

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If a DNA strand has sequence 3’TAACGCTTGGCCAAGTCAGTCAG 5’

Design the primer for the strand has polarity 3’ to 5’

A.      5’ ATTGC 3’

B.      5’ TAAGC 3’

C.      3’ ATTGC 5’

D.     3’ TAAGC 5’

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If a DNA strand has to undergo denaturation in PCR

A.      Need to give optimal conditions

B.      Temperature should be above 90 degree Celsius

C.      Need to give Taq polymerase

D.     A strand cannot undergo denaturation because of absence of hydrogen bonds

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If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?

A.      10240

B.      1024

C.      512

D.     5120

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