The process of competent cell formation include

A.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

B.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

C.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

D.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

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