The process of competent cell formation include

A.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

B.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

C.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

D.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

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Micro-injection is a method of introducing alien DNA into

A.      Cytoplasm of all cells

B.      Cytoplasm of bacterial cell

C.      Nucleus of animal cell

D.     Nucleus of plant cell

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Biolistics

A.      Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA

B.      Also known as gene gun

C.      Both A and B

D.     Direct injection

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pBR322 means

A.      BR for the initials of two scientists who constructed it

B.      BR for the resistance of BamH1

C.      BR for the multiple cloning site

D.     BR  for its discovery in Berlin

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The antibiotic resistance gene in pBR322 is not

A.      Tetracycline

B.      Chloramphenicol

C.      Ampicillin

D.     Both A and B

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rop segment codes for pBR322?

A.      Resistance protein

B.      Protein for replication

C.      Proteins for translation

D.     Cloning site

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Pvu II restriction site is present in pBR322?

A.      rop

B.      Tetracycline resistance gene

C.      Ampicillin resistance gene

D.     Ori

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BamHI restriction site is present in pBR322?

A.      rop

B.      Tetracycline resistance gene

C.      Ampicillin resistance gene

D.     Ori

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Sal I restriction site is present in pBR322?

A.      rop

B.      Tetracycline resistance gene

C.      Ampicillin resistance gene

D.     Ori

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Pvu I restriction site is present in pBR322?

A.      rop

B.      Tetracycline Resistance gene

C.      Ampicillin Resistance gene

D.     Ori

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