In order to make the host cell competent
Divalent cations such as calcium are used-
A. The divalent ions has to be in specific concentration
B. They cause the DNA uptake by the cell
C. They increase the efficiency with which DNA enters the bacterium through pores in its cell membrane.
D. Both B and C
NCERT page number 200
11.2 Tools of RDT
11.2.3 Competent host (For….
Biotechnology: Principles And Processes