For easiest cloning, one needs to get vector with

A.      Single cloning site

B.      Multiple cloning site

C.      Few cloning site

D.     No cloning site at all

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If the gene of Beta galactosidase is not inactivated, means there will be color in the colonies of

A.      Non- recombinant

B.      Recombinant

C.      Transformants

D.     Non-transformants

Concept Questions :-

Selectable Markers:III
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Insertional inactivation results in

A.      Non-recombinants

B.      Recombinants

C.      Transformants

D.     Non- transformants

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Agrobacterium tumifaciens

A.      A pathogen of  angiosperms

B.      It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

C.      Ti plasmid of it is now modified into an expression vector

D.     It's Ti plasmid is modified into a vector which is no more pathogenic

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In order to make the host cell competent

Divalent cations such as calcium are used-

A.      The divalent ions has to be in specific concentration

B.      They cause the DNA uptake by the cell

C.      They increase the efficiency with which DNA enters the bacterium through pores in its cell membrane.

D.     Both B and C

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The process of competent cell formation include

A.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

B.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

C.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

D.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

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Micro-injection is a method of introducing alien DNA into

A.      Cytoplasm of all cells

B.      Cytoplasm of bacterial cell

C.      Nucleus of animal cell

D.     Nucleus of plant cell

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Biolistics

A.      Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA

B.      Also known as gene gun

C.      Both A and B

D.     Direct injection

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pBR322 means

A.      BR for the initials of two scientists who constructed it

B.      BR for the resistance of BamH1

C.      BR for the multiple cloning site

D.     BR  for its discovery in Berlin

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The antibiotic resistance gene in pBR322 is not

A.      Tetracycline

B.      Chloramphenicol

C.      Ampicillin

D.     Both A and B

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