Which of the following is not a feature to facilitate cloning into a vector?

A.      Origin of replication

B.      Selectable Marker

C.      Multiple cloning site

D.     Virulence region

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If I want to check the expression of my gene, then I should use

A.      Vectors supporting high copy number

B.      Vectors supporting low copy number

C.      No need to bother about copy number

D.     Vectors with strong Ori

Concept Questions :-

Tools: Vectors:II
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Selectable marker  helps

A.      To identify transformants

B.      To identify recombinants

C.      To identify non- recombinants

D.     To eliminate transformants

Concept Questions :-

Selectable Markers: I
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The normal E. coli cells have

A.      Kanamycin

B.      Tetracycline

C.      Ampicillin

D.     No resistance

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For easiest cloning, one needs to get vector with

A.      Single cloning site

B.      Multiple cloning site

C.      Few cloning site

D.     No cloning site at all

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If the gene of Beta galactosidase is not inactivated, means there will be color in the colonies of

A.      Non- recombinant

B.      Recombinant

C.      Transformants

D.     Non-transformants

Concept Questions :-

Selectable Markers:III
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Insertional inactivation results in

A.      Non-recombinants

B.      Recombinants

C.      Transformants

D.     Non- transformants

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Agrobacterium tumifaciens

A.      A pathogen of  angiosperms

B.      It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

C.      Ti plasmid of it is now modified into an expression vector

D.     It's Ti plasmid is modified into a vector which is no more pathogenic

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In order to make the host cell competent

Divalent cations such as calcium are used-

A.      The divalent ions has to be in specific concentration

B.      They cause the DNA uptake by the cell

C.      They increase the efficiency with which DNA enters the bacterium through pores in its cell membrane.

D.     Both B and C

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The process of competent cell formation include

A.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

B.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

C.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

D.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

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