Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Subtopic:  Tools: Enzymes: I | Tools |
 58%
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Which of the following steps will be performed after restriction digestion?

1.      Ligation

2.      Elution from DNA gel

3.      Transformation

4.      Cloning

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
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If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

1. a- bacteriophage

2. a- Plasmid

3. a- bacteriophage or Plasmid

4. a- prion

Subtopic:  Tools: Vectors: II | Tools |
 64%
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Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

Subtopic:  Tools: Vectors: I | Tools: Vectors: II | Tools |
 69%
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If I want to check the expression of my gene, then I should use

1.      Vectors supporting high copy number

2.      Vectors supporting low copy number

3.      No need to bother about copy number

4.     Vectors with strong Ori

Subtopic:  Tools: Vectors: II | Tools |
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Selectable marker helps

1.      To identify transformants

2.      To identify recombinants

3.      To identify non- recombinants

4.      To eliminate transformants

Subtopic:  Selectable Markers: I | Selectable Markers: II | Selectable Markers |
 55%
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The normal E. coli cells have

1.      Kanamycin

2.      Tetracycline

3.      Ampicillin

4.     No resistance

Subtopic:  Host & Desired DNA: I | Host & Desired DNA |
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For easiest cloning, one needs to get vector with

1.      Single cloning site

2.      Multiple cloning site

3.      Few cloning site

4.     No cloning site at all

Subtopic:  Tools: Vectors: I | Tools |
 67%
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If the gene of Beta-galactosidase is not inactivated, means there will be color in the colonies of

1.      Non- recombinant

2.      Recombinant

3.      Transformants

4.     Non-transformants

Subtopic:  Selectable Markers: III | Selectable Markers |
 60%
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Insertional inactivation results in

1.      Non-recombinants

2.      Recombinants

3.      Transformants

4.     Non- transformants

Subtopic:  Selectable Markers: III | Selectable Markers |
 72%
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