Which of the following step is last for isolating the desired DNA fragment?

A.      Running of gel

B.      Elution

C.      Staining

D.     Visualization

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Arrange the following steps in order:

1) Restriction Digestion 2) Running Agarose gel 3) Elution of bands 4) Purification of DNA 5)ligation

6) transformation 7) Cloning

A.      1-2-3-4-5-6-7

B.      1-5-6-7-2-3-4

C.      1-2-3-5-6-7-4

D.     2-3-4-1-5-6-7

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Which of the following steps will be performed after restriction digestion?

A.      Ligation

B.      Elution from DNA gel

C.      Transformation

D.     Cloning

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If one performs an experiment of restriction digestion and then without separating bands from DNA goes for ligation reaction. There will be

A.      Non specific ligations

B.      Undesired products

C.      No ligation

D.     Both A And B

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In a DNA gel the fragment of 2kb and 4kb will be

A.      2kb nearer to cathode while 4kb nearer to anode

B.      2kb closer to wells while 3kb towards the opposite ends

C.      2kb closer to opposite end of gel while 3kb closer to well

D.     2kb away from cathode while 3kb towards anode

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If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

A.      a- bacteriophage

B.      a- Plasmid

C.      a- bacteriophage or Plasmid

D.     a- prion

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Which of the following is not a feature to facilitate cloning into a vector?

A.      Origin of replication

B.      Selectable Marker

C.      Multiple cloning site

D.     Virulence region

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If I want to check the expression of my gene, then I should use

A.      Vectors supporting high copy number

B.      Vectors supporting low copy number

C.      No need to bother about copy number

D.     Vectors with strong Ori

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Selectable marker  helps

A.      To identify transformants

B.      To identify recombinants

C.      To identify non- recombinants

D.     To eliminate transformants

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The normal E. coli cells have

A.      Kanamycin

B.      Tetracycline

C.      Ampicillin

D.     No resistance

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