Arrange the following enzymes/ processes in the order of their use for a certain experiment of genetic engineering:

1) ligase, 2) restriction enzyme 3)desired gene 4) Transformation 5) Expression

A.      3-2-1-4-5

B.      1-2-4-5-3

C.      3-1-4-5-2

D.     2-5-4-3-1

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