Insertional inactivation of the lac Z gene forms-

(1) Blue recombinant colonies

(2) Colourless recombinant colonies

(3) Fluorescent green colonies

(4) There is no relation between the lac Z gene and colour of the colony.

Subtopic:  Selectable Markers: III | Selectable Markers |
 70%
From NCERT
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A reporter enzyme used in biotechnology is-

(1) Green Fluorescent Protein

(2) Luciferase

(3) b Galactosidase

(4) All of the above

Subtopic:  Selectable Markers: III | Selectable Markers |
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Downstream processing does not include

(1) Quality control

(2) Clinical trials

(3) Product purification

(4) Sampling from bioreactor

Subtopic:  Large Scale Production: II | Large Scale Production |
 64%
From NCERT
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Which is not a component of the vector pBR322?

(1) Chloramphenicol selectable marker

(2) Replication proteins

(3) Origin of replication

(4) Cloning site for BamHI enzyme

Subtopic:  Tools: Vectors: II | Tools |
 75%
From NCERT
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Which among the following is a cloning vector?

(1) EcoRI

(2) BamHI

(3) pUC18

(4) Sal I

Subtopic:  Tools: Vectors: II | Tools |
From NCERT
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Two bacteria found to be very useful in genetic engineering are

(1) Diplococcus and Streptomyces

(2) Rhizobium and Xanthomonas

(3) Vibrio and Escherischia

(4) Escherischia and Agrobacterium

Subtopic:  Tools: Vectors: II | Host & Desired DNA: I | Host & Desired DNA | Tools |
 97%
From NCERT
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Which of the following would you choose as the safest and least cumbersome selectable marker-

(1) Ampicillin resistance gene

(2) Tetracyclin resistance gene

(3) Kanamycin resistance gene

(4) b Galactosidase gene

Subtopic:  Selectable Markers: III | Selectable Markers |
 68%
From NCERT
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Recombinant DNA is formed in nature during-

(1) Crossing over at pachytene

(2) Integration of plasmid to form episome

(3) Integration of phage DNA to form prophage

(4) More than one is correct.

Subtopic:  Tools: Vectors: I | Tools |
 69%
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Southern Blotting essentially makes use of a

(1) Buffer

(2) Electrodes

(3) Agarose gel

(4) All of the above

Subtopic:  Process of Biotech |
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The native charge on DNA molecules is

(1) Positive

(2) Negative

(3) Neutral

(4) DNA is an ampholyte

Subtopic:  Process of Biotech |
 95%
From NCERT
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