Insertional inactivation of the lac Z gene forms-

  1. Blue recombinant colonies
  2. Colourless recombinant colonies
  3. Fluorescent green colonies
  4. There is no relation between the lac Z gene and colour of the colony.
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A reporter enzyme used in biotechnology is-

  1. Green Fluorescent Protein
  2. Luciferase
  3. b Galactosidase
  4. All of the above
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Downstream processing does not include

  1. Quality control
  2. Clinical trials
  3. Product purification
  4. Sampling from bioreactor
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Probes are molecules of

  1. DNA
  2. RNA
  3. Proteins
  4. All of the above
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Which is not a component of the vector pBR322?

  1. Chloramphenicol selectable marker
  2. Replication proteins
  3. Origin of replication
  4. Cloning site for BamHI enzyme
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Genetic engineering is possible because-

  1. DNA can be cut at specific sites by using enzymes like DNAase II
  2. Restriction enzymes can be used in vitro
  3. DNA ligase can join two pieces of DNA
  4. E.coli acts as an ideal host for cloning.
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Which among the following is a cloning vector?

  1. EcoRI
  2. BamHI
  3. pUC18
  4. Sal I
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Two bacteria found to be very useful in genetic engineering are

  1. Diplococcus and Streptomyces
  2. Rhizobium and Xanthomonas
  3. Vibrio and Escherischia
  4. Escherischia and Agrobacterium
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Which of the following is a recombinant protein-

  1. b Galactosidase in E.coli
  2. Insulin in humans
  3. Somatotropin in humans
  4. Interferon in E. coli
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An example of a transgenic is

  1. E.coli expressing b Galactosidase gene
  2. Carrot expressing Nif genes
  3. HIV expressing Reverse transcriptase gene
  4. Brassica expressing erusic acid gene
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