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Q. No.In agarose gel electrophoresis the separated DNA fragments can be visualized only after staining the DNA with a compound known as:
1. Polyethylene glycol
2. Janus Green
3. DEC
4. Ethidium bromide
Subtopic: Separation and Isolation of DNA fragments |
97%
Level 1: 80%+
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Biotechnology has been defined as ‘the integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services, by:
| 1. | EFB | 2. | American Chemical Society |
| 3. | GEAC | 4. | NCBI |
Subtopic: Introduction & History: I |
90%
Level 1: 80%+
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Identify the correct statements:
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Herbert Boyer performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. |
| II: | Stanley Cohen developed a method of removing plasmids from the bacterial cell and then reinserting them in other bacterial cells. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Introduction & History: I |
72%
Level 2: 60%+
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Maintenance of sterile ambience in chemical engineering processes to enable growth of only the desired microbe/eukaryotic cell in large quantities for the manufacture of biotechnological products like antibiotics, vaccines, enzymes, etc is called as:
| 1. | Genetic engineering | 2. | Bioprocess engineering |
| 3. | Gene technology | 4. | Biopharming |
Subtopic: Introduction & History: II |
85%
Level 1: 80%+
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Restriction enzymes have been isolated from:
I: Bacteria
II: Yeasts
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
I: Bacteria
II: Yeasts
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Restriction Enzymes - Main Enzymes |
74%
Level 2: 60%+
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What can be the natural function of a restriction enzyme?
| 1. | To remove introns from hnRNA |
| 2. | To cut dsRNA resulting in the formation of miRNA |
| 3. | To cut foreign DNA |
| 4. | To join exons after removal of introns from a transcript |
Subtopic: Introduction & History: II |
85%
Level 1: 80%+
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Bacterial cells add methyl groups to their DNA in order to:
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
Subtopic: Restriction Enzymes - Main Enzymes |
89%
Level 1: 80%+
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Resistance to certain antibiotics in bacterial cells:
I: is a genetic trait
II: is normally carried on the main chromosomal DNA
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
I: is a genetic trait
II: is normally carried on the main chromosomal DNA
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Tools: Vectors: I |
51%
Level 3: 35%-60%
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The ‘sticky ends’ produced in the fragments of DNA produced by some restriction enzymes are composed of:
| 1. | methyl groups | 2. | telomeres |
| 3. | unpaired bases | 4. | Guanine rich repeats |
Subtopic: Restriction Enzymes - Main Enzymes |
71%
Level 2: 60%+
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It is usually possible to make bacterial cells more permeable to take up plasmid by treatment with:
1. alkali
2. ultrasound
3. divalent calcium ions
4. trivalent phosphate ions
1. alkali
2. ultrasound
3. divalent calcium ions
4. trivalent phosphate ions
Subtopic: Obtaining Copy of Gene from Donor DNA |
96%
Level 1: 80%+
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