NEET Zoology Biotechnology Principles and Processes Questions Solved

AIIMS - 2012

A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(a) b, a, c, d

(b) a, b, c, d

(c) c, b, a, d

(d) b, a, d, c

The positions of these DNA fragments on the electrophoresis gel depends upon relative molecular weight. The fragment with lowest molecular weight will appear farther from the wall and fragment with higher molecular weight will appear near the wall. So on the basis of information given. Molecular weight of d is maximum and molecular weight of b is minimum. So, the order of fragment will be

(b, a, c, d).

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