Biolistic (gene gun) is suitable for

1. disarming pathogen vectors

2. transformation of plant cell

3. constructing recombinant DNA by joining with vectors

4. DNA fingerprinting

Subtopic:  Tools: Vectors: II | Tools |
 80%
From NCERT
AIIMS - 2015
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A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(1) b, a, c, d

(2) a, b, c, d

(3) c, b, a, d

(4) b, a, d, c

Subtopic:  Process of Biotech |
 63%
AIIMS - 2012
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Which of the following DNA sequences qualifies to be designated as a palindrome?

(1) 5 - GACCAG - 3’ in one strand

(2) 3’- GACCAG - 5’ in one strand

(3) 5’- GACGAG- 3’ 3’- CTGGTC - 5’

(4) 5’- AGCGCT - 3’ 3’- TCGCGA - 5’

Subtopic:  Tools: Enzymes: II | Tools |
 83%
From NCERT
AIIMS - 2012
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The source of taq polymerase used in PCR is a

(1) thermophilic fungus

(2) mesophilic fungus

(3) thermophilic bacterium

(4) halophilic bacterium

Subtopic:  Large Scale Production: I | Large Scale Production |
 93%
From NCERT
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pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an

1. original bacterial plasmid

2. modified bacterial plasmid

3. viral genome

4. transposon

Subtopic:  Tools: Vectors: II | Tools |
 71%
AIIMS - 2010
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Which of the following statement is not true: 

(1) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium

(2) Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli

(3)  The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin

(4) Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning

Subtopic:  Tools: Vectors: I | Tools |
 69%
From NCERT
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Go through the statements:

A. During the processing of the prohormone "proinsulin" into the mature "insulin", C-peptide is removed from proinsulin.

B. In hydridoma technology, B-cells are fused with myeloma cells.

C. Genetic engineering has been successfully used for producing transgenic mice for testing safety of polio vaccine before use in humans.

D. Some of the characteristics of Bt cotton are high yield and resistance to bollworms.

E. An improved variety of transgenic basmati rice gives high yield and is rich in vitamin A.

F. Gray biotechnology' is referred to industrial process.

G. The polymerase chain reaction is a technique that is used for in vivo replication of DNA. 

How many statement(s) is wrong?

(1) One

(2) Two

(2) Four

(4) Six

Subtopic:  Large Scale Production: II | Large Scale Production |
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Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be:

A. Denatured double stranded DNA probes

B. doubles stranded RNA probes

C. Protein probes

D. Single stranded DNA probes.

(1) A, B

(2) B, C

(3) A, D

(4) A, B, C, D

Subtopic:  Process of Biotech |
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Which is a natural genetic engineer of the plants?

(1)E.coli

(2)A. tumefaciens

(3)Rhizobium

(4)Pseudomonas

Subtopic:  Tools: Vectors: II | Tools |
 83%
From NCERT
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The thermostable enzyme Taq polymerase used in PCR technique was discovered by

(1)Alec Jeffrey

(2)Karry Mullis

(3)Temin

(4)Baltimore

Subtopic:  Polymerase Chain Reaction: PCR |
 66%
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