Biolistic (gene gun) is suitable for

(a) disarming pathogen vectors

(b) transformation of plant cell

(c) constructing recombinant DNA by joining with vectors

(d) DNA fingerprinting

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A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(a) b, a, c, d

(b) a, b, c, d

(c) c, b, a, d

(d) b, a, d, c

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Which of the following DNA sequences qualifies to be designated as a palindrome?

(a) 5 - GACCAG - 3’ in one strand

(b) 3’- GACCAG - 5’ in one strand

(c) 5’- GACGAG- 3’ 3’- CTGGTC - 5’

(d) 5’- AGCGCT - 3’ 3’- TCGCGA - 5’

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pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an

(a) original bacterial plasmid

(b) modified bacterial plasmid

(c) viral genome

(d) transposon

Concept Questions :-

Tools: Vectors:II
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Which of the following statement is not true: 

(1) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium

(2) Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli

(3)  The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin

(4) Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning

Concept Questions :-

Tools: Vectors:II
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Go through the statements:

A. During the processing of the prohormone "proinsulin" into the mature "insulin", C-peptide is removed from proinsulin.

B. In hydridoma technology, B-cells are fused with myeloma cells.

C. Genetic engineering has been successfully used for producing transgenic mice for testing safety of polio vaccine before use in humans.

D. Some of the characteristics of Bt cotton are high yield and resistance to bollworms.

E. An improved variety of transgenic basmati rice gives high yield and is rich in vitamin A.

F. Gray biotechnology' is referred to industrial process.

G. The polymerase chain reaction is a technique that is used for in vivo replication of DNA. 

How many statement(s) is wrong?

(1) One

(2) Two

(2) Four

(4) Six

Concept Questions :-

Large Scale Production: II
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Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be:

A. Denatured double stranded DNA probes

B. doubles stranded RNA probes

C. Protein probes

D. Single stranded DNA probes.

(1) A, B

(2) B, C

(3) A, D

(4) A, B, C, D

Concept Questions :-

Process of Biotech
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A gene from one organism joined to the regulatory sequence of another organism makes a

    1. Junk DNA
    2. Chimeric DNA
    3. Recombinant DNA
    4. More than one is correct

Concept Questions :-

Intro & History: II
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To be useful in genetic engineering a vector must have

    1. An origin for replication
    2. Low copy number
    3. Low rates of multiplication
    4. Many cloning sites for the same enzyme
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In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was

    1. Methylase
    2. Exonuclease
    3. Aminotransferase
    4. Terminal Transferase
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