Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the potions given.
A. Electrophoresis of DNA fragments
B. Hybridisation with DNA probe
C. Digestion of DNA by restriction endonucleases
E. Blotting of NDA fragments to nitrocellulose membrane
(1) C – A – B – E – D
(2) C – A – E – B – D
(3) A – E – C – B – D
(4) A – C – E – D – B
(2) The technique of DNA fingerprinting involves following steps:
(i) Isolation of DNA from sample cell.
(ii) Amplification of DNA using polymerase chain reaction (PCR), (if DNA is less in amount).
(iii) Digestion of this DNA by restriction endonucleases (C).
(iv) These DNA fragments are electrophoresed in agarose gel. These fragments can be visualised using EtBr-UV system (A).
(v) Separated DNA fragments are transferred to nitrocellulose membrane using Southern Blotting (E).
(vi) Probing for VNTRs is done using labelled DNA probes (B).
(vii) The hybridised fragment can be detected by autoradiography (D).