NEET Zoology Biotechnology Principles and Processes Questions Solved

AIIMS - 2015

Select the correct order of processing of PCR.

(a) Extension, primer annealing, denaturation

(b) Denaturation, primer annealing, extension

(c) Denaturation, extension, primer annealing

(d) Primer annealing, denaturation, extension

(b) Polymerase chain reaction (PCR) is a technique to amplify a selected DNA sequence by a million fold or more. In this procedure, the DNA containing the sequence to be amplified is denatured by heating. This denatured DNA is then annealed to the synthetic oligonucleotide primers. Then Taq polymerase is used to replicate the DNA segment between the sites complementary to oligonucleotide primer and extend it.

Difficulty Level:

  • 5%
  • 77%
  • 13%
  • 7%