Select the correct order of processing of PCR.

(a) Extension, primer annealing, denaturation

(b) Denaturation, primer annealing, extension

(c) Denaturation, extension, primer annealing

(d) Primer annealing, denaturation, extension

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Plasmid vector in DNA recombinant technology means

(a) a virus that transfers gene to bacteria

(b) extra-chromosomal autonomously replicating circular DNA

(c) sticky end of DNA

(d) any fragment of DNA carrying desirable gene

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Which organism is used to transfer T-DNA?

(a) Streplomyces hygroscopicus

(b) Agrobacterium tumefaciens

(c) Salmonella typhi

(d) Escherichia coli

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Match the following column I with column II.

Column I                                      Column II

A. Agrostology                              1. Earth

B. Smallest flowering plant            2. Genetic engineering

C. Tectonic                                   3.Migration

D. Ecesis                                      4.Wolffia

E. Euphanix                                  5.Grass

Codes

       A     B     C     D     E

(a)   5     4     1     3     2   

(b)   1     2     3     4     5

(c)   2     3     1     5     4

(d)   5     4     3     2     1

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Biolistic (gene gun) is suitable for

(a) disarming pathogen vectors

(b) transformation of plant cell

(c) constructing recombinant DNA by joining with vectors

(d) DNA fingerprinting

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A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(a) b, a, c, d

(b) a, b, c, d

(c) c, b, a, d

(d) b, a, d, c

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Which of the following DNA sequences qualifies to be designated as a palindrome?

(a) 5 - GACCAG - 3’ in one strand

(b) 3’- GACCAG - 5’ in one strand

(c) 5’- GACGAG- 3’ 3’- CTGGTC - 5’

(d) 5’- AGCGCT - 3’ 3’- TCGCGA - 5’

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pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an

(a) original bacterial plasmid

(b) modified bacterial plasmid

(c) viral genome

(d) transposon

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A gene from one organism joined to the regulatory sequence of another organism makes a

    1. Junk DNA
    2. Chimeric DNA
    3. Recombinant DNA
    4. More than one is correct
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To be useful in genetic engineering a vector must have

    1. An origin for replication
    2. Low copy number
    3. Low rates of multiplication
    4. Many cloning sites for the same enzyme
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