Which of the following is not a characteristic of pBR322 vector?
(a) It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.
(b) It is the most widely used, versatile and easily manipulated vector.
(c) It has two antibiotic resistance genes, tetR and ampR.
(d) It does not have restriction site for Sal I.
Select the correct order of processing of PCR.
(a) Extension, primer annealing, denaturation
(b) Denaturation, primer annealing, extension
(c) Denaturation, extension, primer annealing
(d) Primer annealing, denaturation, extension
Plasmid vector in DNA recombinant technology means
(a) a virus that transfers gene to bacteria
(b) extra-chromosomal autonomously replicating circular DNA
(c) sticky end of DNA
(d) any fragment of DNA carrying desirable gene
Which organism is used to transfer T-DNA?
(a) Streplomyces hygroscopicus
(b) Agrobacterium tumefaciens
(c) Salmonella typhi
(d) Escherichia coli
Match the following column I with column II.
Column I Column II
A. Agrostology 1. Earth
B. Smallest flowering plant 2. Genetic engineering
C. Tectonic 3.Migration
D. Ecesis 4.Wolffia
E. Euphanix 5.Grass
A B C D E
(a) 5 4 1 3 2
(b) 1 2 3 4 5
(c) 2 3 1 5 4
(d) 5 4 3 2 1
Biolistic (gene gun) is suitable for
(a) disarming pathogen vectors
(b) transformation of plant cell
(c) constructing recombinant DNA by joining with vectors
(d) DNA fingerprinting
A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be
(a) b, a, c, d
(b) a, b, c, d
(c) c, b, a, d
(d) b, a, d, c
Which of the following DNA sequences qualifies to be designated as a palindrome?
(a) 5 - GACCAG - 3’ in one strand
(b) 3’- GACCAG - 5’ in one strand
(c) 5’- GACGAG- 3’ 3’- CTGGTC - 5’
(d) 5’- AGCGCT - 3’ 3’- TCGCGA - 5’
pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an
(a) original bacterial plasmid
(b) modified bacterial plasmid
(c) viral genome
Concept Questions :-
Which of the following statement is not true:
(1) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium
(2) Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli
(3) The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin
(4) Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning
Concept Questions :-