Which of the following is a palindromic sequence?

(1) 5'-CGTATG-3'           

      3'-CGAATG-5'

(2) 5'-CGAATG-3'

     3'-GCATAC-5'                 

(3) 5'-GAATTC-3'

     3'-CTTAAG-5'      

(4) 5'-GACTAC-3'

     3'-CTTAAG-5'      

How are transformants selected from nontransformants?

Which of the following is not a characteristic of pBR322 vector? 

1. It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.

2. It is the most widely used, versatile and easily manipulated vector.

3. It has two antibiotic resistance genes, tet^R and amp^R.

4. It does not have restriction site for Sal I.

Select the correct order of processing of PCR.

(1) Extension, primer annealing, denaturation

(2) Denaturation, primer annealing, extension

(3) Denaturation, extension, primer annealing

(4) Primer annealing, denaturation, extension

Plasmid vector in DNA recombinant technology means

Which organism is used to transfer T-DNA?

1. Streplomyces hygroscopicus
2. Agrobacterium tumefaciens
3. Salmonella typhi
4. Escherichia coli

Match the following column I with column II.

Column I                                      Column II

A. Agrostology                              1. Earth

B. Smallest flowering plant            2. Genetic engineering

C. Tectonic                                   3.Migration

D. Ecesis                                      4.Wolffia

E. Euphanix                                  5.Grass

Codes

       A     B     C     D     E

(a)   5     4     1     3     2   

(b)   1     2     3     4     5

(c)   2     3     1     5     4

(d)   5     4     3     2     1

Biolistic (gene gun) is suitable for

A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(1) b, a, c, d

(2) a, b, c, d

(3) c, b, a, d

(4) b, a, d, c

Which of the following DNA sequences qualifies to be designated as a palindrome?

1. 5 - GACCAG - 3’ in one strand

2. 3’- GACCAG - 5’ in one strand

3. 5’- GACGAG- 3’ 3’- CTGGTC - 5’

4. 5’- AGCGCT - 3’ 3’- TCGCGA - 5’