Which of the following is a cloning vector?
(a) DNA of Salmonella typhimurium
(b) Ti plasmid
(c) Amp' and Tet' loci
(d) Ori minus PBR322
Which of the following is a palindromic sequence?
(a) 5'-CGTATG-3' (b) 5'-CGAATG-3'
(C) 5'-GAATTC-3' (d) 5'-GACTAC-3'
How are transformants selected from nontransformants?
(a) Presence of more than one recognition site in the vector DNA.
(b) Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker.
(c) Antibiotic resistance gene gets inactivated due to insertion of alien DNA.
(d) Both (b) and (c).
Which of the following is not a characteristic of pBR322 vector?
(a) It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.
(b) It is the most widely used, versatile and easily manipulated vector.
(c) It has two antibiotic resistance genes, tetR and ampR.
(d) It does not have restriction site for Sal I.
Select the correct order of processing of PCR.
(a) Extension, primer annealing, denaturation
(b) Denaturation, primer annealing, extension
(c) Denaturation, extension, primer annealing
(d) Primer annealing, denaturation, extension
Plasmid vector in DNA recombinant technology means
(a) a virus that transfers gene to bacteria
(b) extra-chromosomal autonomously replicating circular DNA
(c) sticky end of DNA
(d) any fragment of DNA carrying desirable gene
Which organism is used to transfer T-DNA?
(a) Streplomyces hygroscopicus
(b) Agrobacterium tumefaciens
(c) Salmonella typhi
(d) Escherichia coli
Match the following column I with column II.
Column I Column II
A. Agrostology 1. Earth
B. Smallest flowering plant 2. Genetic engineering
C. Tectonic 3.Migration
D. Ecesis 4.Wolffia
E. Euphanix 5.Grass
A B C D E
(a) 5 4 1 3 2
(b) 1 2 3 4 5
(c) 2 3 1 5 4
(d) 5 4 3 2 1
Biolistic (gene gun) is suitable for
(a) disarming pathogen vectors
(b) transformation of plant cell
(c) constructing recombinant DNA by joining with vectors
(d) DNA fingerprinting
A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be
(a) b, a, c, d
(b) a, b, c, d
(c) c, b, a, d
(d) b, a, d, c