Hirudin is

(1) a protein produced by Hordeum vulgare, which is rich in lysine

(2) a toxic molecule isolated from Gossypium hirsutum, which reduces human fertility 

(3) a protein produced from transgenic Brassica napus, which prevents blood clotting

(4) an antibiotic produced by a genetically enginnered bacterium, Escherichia coli 

Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?

(1) Lysozyme, lipases, trypsin

(2) Chitinase, cellulase, lysozyme

(3) Chitinase, cellulase, trypsin

(4) Trypsin, lipases, cellulase

What is the source of EcoRI?

1. Escherichia coli R 1
2. Escherichia coli R I 13
3. Escherichia coli R Y 13
4. Escherichia coli R X 13

Which of the following is a cloning vector?

Which of the following is a palindromic sequence?

(1) 5'-CGTATG-3'           

      3'-CGAATG-5'

(2) 5'-CGAATG-3'

     3'-GCATAC-5'                 

(3) 5'-GAATTC-3'

     3'-CTTAAG-5'      

(4) 5'-GACTAC-3'

     3'-CTTAAG-5'      

How are transformants selected from nontransformants?

Which of the following is not a characteristic of pBR322 vector? 

1. It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.

2. It is the most widely used, versatile and easily manipulated vector.

3. It has two antibiotic resistance genes, tet^R and amp^R.

4. It does not have restriction site for Sal I.

Select the correct order of processing of PCR.

(1) Extension, primer annealing, denaturation

(2) Denaturation, primer annealing, extension

(3) Denaturation, extension, primer annealing

(4) Primer annealing, denaturation, extension

Plasmid vector in DNA recombinant technology means

Which organism is used to transfer T-DNA?

1. Streplomyces hygroscopicus
2. Agrobacterium tumefaciens
3. Salmonella typhi
4. Escherichia coli